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Silk scaffolds support culture of live pBMAds. Confocal maximum projection imaging of 3D scaffolds with pBMAds (A) after 3 weeks of culture stained with live-dead (Calcein AM, green; EthD-1; dead) stain. Lipids also appear red and scaffold autofluorescence can appear purple or pink colored. Scale bar, 100 µm. Two representative images shown. (B) Live pBMAds cultured on scaffolds can be quantified with <t>RealTime-Glo</t> (RTG). RTG reagent was seeded with adipocytes on day 0 and relative luciferase activity (RLU) measured for up to 7 days using a plate reader. RTG activity from pBMAds from three donors are quantified here, with background luciferase activity measured in empty scaffolds (n = 3); error bars in donors R22-0211 and R22-0353 represent S.E.M. of RLU from multiple scaffolds (i.e., technical replicates).
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Overall agreement between clinician-collected cervical and self-collected vaginal/urine samples
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Relative Sensitivities of 8 FDA <t> EUA </t> SARS-CoV-2 Molecular Test Platforms.
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Image Search Results


Silk scaffolds support culture of live pBMAds. Confocal maximum projection imaging of 3D scaffolds with pBMAds (A) after 3 weeks of culture stained with live-dead (Calcein AM, green; EthD-1; dead) stain. Lipids also appear red and scaffold autofluorescence can appear purple or pink colored. Scale bar, 100 µm. Two representative images shown. (B) Live pBMAds cultured on scaffolds can be quantified with RealTime-Glo (RTG). RTG reagent was seeded with adipocytes on day 0 and relative luciferase activity (RLU) measured for up to 7 days using a plate reader. RTG activity from pBMAds from three donors are quantified here, with background luciferase activity measured in empty scaffolds (n = 3); error bars in donors R22-0211 and R22-0353 represent S.E.M. of RLU from multiple scaffolds (i.e., technical replicates).

Journal: Frontiers in Oncology

Article Title: Development and characterization of three cell culture systems to investigate the relationship between primary bone marrow adipocytes and myeloma cells

doi: 10.3389/fonc.2022.912834

Figure Lengend Snippet: Silk scaffolds support culture of live pBMAds. Confocal maximum projection imaging of 3D scaffolds with pBMAds (A) after 3 weeks of culture stained with live-dead (Calcein AM, green; EthD-1; dead) stain. Lipids also appear red and scaffold autofluorescence can appear purple or pink colored. Scale bar, 100 µm. Two representative images shown. (B) Live pBMAds cultured on scaffolds can be quantified with RealTime-Glo (RTG). RTG reagent was seeded with adipocytes on day 0 and relative luciferase activity (RLU) measured for up to 7 days using a plate reader. RTG activity from pBMAds from three donors are quantified here, with background luciferase activity measured in empty scaffolds (n = 3); error bars in donors R22-0211 and R22-0353 represent S.E.M. of RLU from multiple scaffolds (i.e., technical replicates).

Article Snippet: For investigation of cell viability over time in our 3D scaffolds, we used two products from Promega: RealTime-Glo and CellTiter-Glo.

Techniques: Imaging, Staining, Cell Culture, Luciferase, Activity Assay

Overall agreement between clinician-collected cervical and self-collected vaginal/urine samples

Journal: Cancer Research and Treatment : Official Journal of Korean Cancer Association

Article Title: Performance and Diagnostic Accuracy of Human Papillomavirus Testing on Self-Collected Urine and Vaginal Samples in a Referral Population

doi: 10.4143/crt.2020.1165

Figure Lengend Snippet: Overall agreement between clinician-collected cervical and self-collected vaginal/urine samples

Article Snippet: Conflicts of Interest The authors from Sejong Medical Co. are employees of and/or shareholders of the company, which developed the RealTime HR-S HPV assay.

Techniques:

Agreement between clinician-collected cervical and self-collected vaginal/urine samples according to age groups

Journal: Cancer Research and Treatment : Official Journal of Korean Cancer Association

Article Title: Performance and Diagnostic Accuracy of Human Papillomavirus Testing on Self-Collected Urine and Vaginal Samples in a Referral Population

doi: 10.4143/crt.2020.1165

Figure Lengend Snippet: Agreement between clinician-collected cervical and self-collected vaginal/urine samples according to age groups

Article Snippet: Conflicts of Interest The authors from Sejong Medical Co. are employees of and/or shareholders of the company, which developed the RealTime HR-S HPV assay.

Techniques: Comparison

Agreement between cervical and vaginal/urine samples according to cytology

Journal: Cancer Research and Treatment : Official Journal of Korean Cancer Association

Article Title: Performance and Diagnostic Accuracy of Human Papillomavirus Testing on Self-Collected Urine and Vaginal Samples in a Referral Population

doi: 10.4143/crt.2020.1165

Figure Lengend Snippet: Agreement between cervical and vaginal/urine samples according to cytology

Article Snippet: Conflicts of Interest The authors from Sejong Medical Co. are employees of and/or shareholders of the company, which developed the RealTime HR-S HPV assay.

Techniques:

Clinical performance of  HPV  test to detect CIN2+ in clinician-collected cervical, self-collected vaginal and urine samples

Journal: Cancer Research and Treatment : Official Journal of Korean Cancer Association

Article Title: Performance and Diagnostic Accuracy of Human Papillomavirus Testing on Self-Collected Urine and Vaginal Samples in a Referral Population

doi: 10.4143/crt.2020.1165

Figure Lengend Snippet: Clinical performance of HPV test to detect CIN2+ in clinician-collected cervical, self-collected vaginal and urine samples

Article Snippet: Conflicts of Interest The authors from Sejong Medical Co. are employees of and/or shareholders of the company, which developed the RealTime HR-S HPV assay.

Techniques:

Relative sensitivity/specificity of  HPV  test to detect CIN2+ in self-collected vaginal and urine samples compared with clinician-collected cervical samples

Journal: Cancer Research and Treatment : Official Journal of Korean Cancer Association

Article Title: Performance and Diagnostic Accuracy of Human Papillomavirus Testing on Self-Collected Urine and Vaginal Samples in a Referral Population

doi: 10.4143/crt.2020.1165

Figure Lengend Snippet: Relative sensitivity/specificity of HPV test to detect CIN2+ in self-collected vaginal and urine samples compared with clinician-collected cervical samples

Article Snippet: Conflicts of Interest The authors from Sejong Medical Co. are employees of and/or shareholders of the company, which developed the RealTime HR-S HPV assay.

Techniques:

Relative Sensitivities of 8 FDA  EUA  SARS-CoV-2 Molecular Test Platforms.

Journal: Journal of Clinical Virology

Article Title: Comparison of the analytical sensitivity of seven commonly used commercial SARS-CoV-2 automated molecular assays

doi: 10.1016/j.jcv.2020.104578

Figure Lengend Snippet: Relative Sensitivities of 8 FDA EUA SARS-CoV-2 Molecular Test Platforms.

Article Snippet: Utilizing SARS-CoV-2 droplet digital PCR (ddPCR) [ ] quantified single positive clinical specimen, we compared the analytical sensitivity of seven SARS-CoV-2 EUA-granted assays, the Abbott Molecular RealTime, the NeuMoDx™, the Roche Cobas®, the BD SARS-CoV-2 reagents for BD MAX™ system, the Hologic Aptima®, the Xpert Xpress, and the GenMark ePlex SARS-CoV-2 tests.

Techniques: Concentration Assay

A literature and manufacturer based summary of sensitivity data.

Journal: Journal of Clinical Virology

Article Title: Comparison of the analytical sensitivity of seven commonly used commercial SARS-CoV-2 automated molecular assays

doi: 10.1016/j.jcv.2020.104578

Figure Lengend Snippet: A literature and manufacturer based summary of sensitivity data.

Article Snippet: Utilizing SARS-CoV-2 droplet digital PCR (ddPCR) [ ] quantified single positive clinical specimen, we compared the analytical sensitivity of seven SARS-CoV-2 EUA-granted assays, the Abbott Molecular RealTime, the NeuMoDx™, the Roche Cobas®, the BD SARS-CoV-2 reagents for BD MAX™ system, the Hologic Aptima®, the Xpert Xpress, and the GenMark ePlex SARS-CoV-2 tests.

Techniques: In Vitro, Cell Culture